只需 5 分钟,ExoSAP-IT™
ExpressPCR Product Cleanup Reagent 即可从 PCR 反应中除去过量的引物和未结合的核苷酸。与替代纯化方法（如离心柱或微珠）相比,该酶法纯化方法是一种更精确、产量更高和周转时间更快的方法。
• Remove unincorporated primers and nucleotides in about 5 minutes
• Prepare DNA sequencing samples with one pipetting step
• Conserve precious PCR products with up to 99% recovery, regardless of amplicon length
• Minimize spin column or bead cleanup
ExoSAP-IT ExpressPCR Product Cleanup Reagent consists of two hydrolytic enzymes: a novel exonuclease I and shrimp alkaline phosphatase (SAP), in a specially formulated buffer.反应设置仅需一个移液步骤即可完成,其后是两次短暂孵育。次孵育可以水解过量的引物并使核苷酸去磷酸化。第二次高温孵育在约一分钟内不可逆地灭活酶。将 ExoSAP-IT Express试剂直接加入 PCR 产物中,可省略向管、孔或柱的转移步骤并节省 PCR 扩增子,从而有助于降低交叉污染的可能性；此外,也无需进一步处理。样品即可用于 DNA 测序或 SNP 分析。
• Remove unincorporated primers and nucleotides in about 5 minutes
• Prepare DNA sequencing samples with one pipetting step
• Conserve precious PCR products with up to 99% recovery, regardless of amplicon length
• Minimize spin column or bead cleanup
ExoSAP-IT ExpressPCR Product Cleanup Reagent consists of two hydrolytic enzymes: a novel exonuclease I and shrimp alkaline phosphatase (SAP), in a specially formulated buffer.反应设置仅需一个移液步骤即可完成,其后是两次短暂孵育。次孵育可以水解过量的引物并使核苷酸去磷酸化。第二次高温孵育在约一分钟内不可逆地灭活酶。将 ExoSAP-IT Express试剂直接加入 PCR 产物中,可省略向管、孔或柱的转移步骤并节省 PCR 扩增子,从而有助于降低交叉污染的可能性；此外,也无需进一步处理。样品即可用于 DNA 测序或 SNP 分析。
采购中心
