5-BrdU(Synonyms:5-溴脱氧尿-嘧啶核苷）
5-BrdU (BrdU) 是一种核苷类似物,可与胸苷竞争掺入 DNA。5-BrdU 通常用于检测增殖细胞。
生物活性
5-BrdU (BrdU) is a nucleoside analog that competes with thymidine for incorporation into DNA.5-BrdU is commonly used in the detection of proliferating cells.
体外研究(In Vitro)
Bromodeoxyuridine induces a progressive, dose-responsive suppression of cancer cell line and cancer stem cell population expansion RG2 rat glioma cells.In H9 cells and BJ fibroblasts, bromodeoxyuridine alters the cell cycle profile[1].BrdU is stably integrated into the DNA, and thus can be used in assessment of cell proliferation and other cell procession[2].
体内研究(In Vivo)
Bromodeoxyuridine (300 mg/kg, i.p.or 0.8 mg/mL, p.o.) significantly slows tumor progression in rat glioma RG2 tumor model[1].
分子量：307.10
Formula：C9H11BrN2O5
CAS 号：59-14-3
中文名称：5-溴脱氧尿-嘧啶核苷
运输条件：Room temperature in continental US!may vary elsewhere.
储存方式：
4°C, protect from light
*In solvent : -80°C, 6 months!-20°C, 1 month (protect from light)
溶解性数据
DMSO : 250 mg/mL(814.07 mM;Need ultrasonic)
H2O : 2 mg/mL(6.51 mM;ultrasonic and warming and heat to 60°C)
| 浓度溶剂体积质量 | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.2563 mL | 16.2813 mL | 32.5627 mL |
| 5 mM | 0.6513 mL | 3.2563 mL | 6.5125 mL |
| 10 mM | 0.3256 mL | 1.6281 mL | 3.2563 mL |
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months!-20°C, 1 month (protect from light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂：
——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存；体内实验的工作液,建议您现用现配,当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶
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1.
请依序添加每种溶剂： PBS
Solubility: 16.25 mg/mL (52.91 mM)!Clear solution!Need ultrasonic
-
2.
请依序添加每种溶剂： 10% DMSO40% PEG3005% Tween-8045% saline
Solubility: ≥ 2.08 mg/mL (6.77 mM)!Clear solution
-
3.
请依序添加每种溶剂： 10% DMSO90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (6.77 mM)!Clear solution
-
4.
请依序添加每种溶剂： 10% DMSO90% corn oil
参考文献
[1].Levkoff LH, et al.Bromodeoxyuridine inhibits cancer cell proliferation in vitro and in vivo.Neoplasia.2008 Aug;10(8):804-16.
[2].Rothaeusler K, et al.Assessment of cell proliferation by 5-bromodeoxyuridine (BrdU) labeling for multicolor flow cytometry.Curr Protoc Cytom.2007 Apr;Chapter 7:Unit7.31
注：产品仅用于科研
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