Melatonin(Synonyms:褪黑素!N-Acetyl-5-methoxytryptamine)
Melatonin 是一种由松果体分泌的ji素,可ji活褪黑ji素受体。Melatonin 在睡眠中起作用并具有重要的抗氧化和抗炎特性。Melatonin 是一种新型的选择性 ATF-6 抑制剂,可通过 COX-2 下调诱导人肝癌细胞凋亡。Melatonin 通过内质网应激减弱palmitic acid-induced (HY-N0830)诱导的小鼠颗粒细胞凋亡。
生物活性
Melatonin is a hormone made by the pineal gland that can activatesmelatonin receptor.Melatonin plays a role in sleep and possesses important antioxidative and anti-inflammatory properties[1][2][3].Melatonin is a novel selectiveATF-6inhibitor and induces human hepatoma cell apoptosis through COX-2 downregulation[4].Melatonin attenuates palmitic acid-induced (HY-N0830) mouse granulosa cells apoptosis via endoplasmic reticulum stress[5].
IC50& Target
Microbial MetaboliteHuman Endogenous Metabolite
体内研究(In Vivo)
Melatonin increases the levels of activated PTEN, RSK-1, mTOR and AMPKα kinases, mildly inhibits ERK-1/2 phosphorylation and Bad phosphorylation, significantly inhibits phosphorylations of S6 Ribosomal Protein, 4E-BP1, GSK-3α and GSK-3β, and slightly increases PRAS40 phosphorylation in animals[1].Melatonin ameliorates the neurotoxiciy and astrocyte activation induced by Aβ1-42in the cerebral cortex.Melatonin also blocks the reduction in Reelin and Dab1 expression induced by Aβ1-42[2].Melatonin treatment and lack of NLRP3-/-share similar inhibition of NF-κB and NLRP3 signaling pathway in mice.Melatonin treatment and lack of NLRP3-/-share some patterns of clock genes expression, and improve cardiomyocytes morphology in mice[3].
分子量：232.28
Formula：C13H16N2O2
CAS 号：73-31-4
中文名称：褪黑素；褪黑激素；美拉酮宁；抑黑素；松果腺素；松果体素
运输条件：Room temperature in continental US!may vary elsewhere.
储存方式
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
溶解性数据
DMSO : 100 mg/mL(430.51 mM;Need ultrasonic)
Ethanol : 12.5 mg/mL(53.81 mM;Need ultrasonic)
H2O : 1.1 mg/mL(4.74 mM;Need ultrasonic)
| 浓度溶剂体积质量 | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 4.3051 mL | 21.5257 mL | 43.0515 mL |
| 5 mM | 0.8610 mL | 4.3051 mL | 8.6103 mL |
| 10 mM | 0.4305 mL | 2.1526 mL | 4.3051 mL |
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months!-20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂：
——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存；体内实验的工作液,建议您现用现配,当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶
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1.
请依序添加每种溶剂： 5% DMSO40% PEG3005% Tween-8050% saline
Solubility: ≥ 2.62 mg/mL (11.28 mM)!Clear solution
-
2.
请依序添加每种溶剂： 5% DMSO95% (20% SBE-β-CD in saline)
Solubility: ≥ 2.62 mg/mL (11.28 mM)!Clear solution
-
3.
请依序添加每种溶剂： 10% DMSO40% PEG3005% Tween-8045% saline
Solubility: ≥ 2.5 mg/mL (10.76 mM)!Clear solution
-
4.
请依序添加每种溶剂： 10% DMSO90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (10.76 mM)!Clear solution
-
5.
请依序添加每种溶剂： 10% DMSO90% corn oil
Solubility: ≥ 2.5 mg/mL (10.76 mM)!Clear solution
-
6.
请依序添加每种溶剂： 10% EtOH40% PEG3005% Tween-8045% saline
Solubility: ≥ 2.5 mg/mL (10.76 mM)!Clear solution
-
7.
请依序添加每种溶剂： 10% EtOH90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (10.76 mM)!Clear solution
-
8.
请依序添加每种溶剂： 10% EtOH90% corn oil
Solubility: ≥ 2.5 mg/mL (10.76 mM)!Clear solution
参考文献
[1]. Kilic U, et al.Particular phosphorylation of PI3K/Akt on Thr308 via PDK-1 and PTEN mediates melatonin's neuroprotective activity after focal cerebral ischemia in mice.Redox Biol.2017 Apr 5;12:657-665
[2]. Hu C, et al.Neuroprotective effect of melatonin on soluble Aβ1-42-induced cortical neurodegeneration via Reelin-Dab1 signaling pathway.Neurol Res.2017 Apr 7:1-1
[3]. Rahim I, et al.Melatonin administration to wild-type mice and non-treated NLRP3 mutant mice share similar inhibition of the inflammatory response during sepsis.J Pineal Res.2017 Mar 31
[4]. Bu LJ, et al.Melatonin, a novel selective ATF-6 inhibitor, induces human hepatoma cell apoptosis through COX-2 downregulation.World J Gastroenterol.2017 Feb 14;23(6):986-998.
[5]. Zhi Chen, et al.Melatonin attenuates palmitic acid-induced mouse granulosa cells apoptosis via endoplasmic reticulum stress.J Ovarian Res.2019 May 10;12(1):43
注：产品仅用于科研
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