Butylhydroxyanisole(Synonyms: 丁基羟基茴香醚!Butylated hydroxyanisole!BHA!E320)
Butylhydroxyanisole (Butylated hydroxyanisole) 是一种抗氧化剂,用作食品防腐剂。Butylhydroxyanisole 能介导肝毒性、生殖qi官发育和学习迟缓以及睡眠不足。Butylhydroxyanisole 具有神经毒性,能导致大脑和神经发育中断。Butylhydroxyanisole 也是一个铁死亡 (ferroptosis) 诱导剂。
生物活性
Butylhydroxyanisole (Butylated hydroxyanisole) is an antioxidant used as a food additive preservative.Butylhydroxyanisole mediates liver toxicity, retardation in reproductive organ development and learning, and sleep deficit.Butylhydroxyanisole exerts neurotoxic effects and leads to disruption of the brain and nerve development[1][2][3].Butylhydroxyanisole is aferroptosisinducer[4].
体外研究(In Vitro)
Butylhydroxyanisole exerts neurotoxic effects by promoting cytosolic calcium accumulation and endoplasmic reticulum stress in astrocytes[1].
Butylhydroxyanisole (25-100 μM!48 hours) inhibits growth and induces death in human astrocytes[1].
Butylhydroxyanisole (100 μM!48 hours) decreases the expression of cell-cycle-related proteins and increased the expression of the cell cycle inhibitory protein[1].
Butylhydroxyanisole (100 μM!48 hours) mediates apoptotic signals in NHA-SV40LT Cells[1].
Butylhydroxyanisole also increases the cytosolic calcium level and the expression of endoplasmic reticulum stress proteins[1].
Butylhydroxyanisole induces testicular dysfunction in mouse testis cells by dysregulating calcium homeostasis and stimulating endoplasmic reticulum stress[2].
MCE has not independently confirmed the accuracy of these methods.They are for reference only.
Cell Proliferation Assay[1]
| Cell Line: | NHA-SV40LT cells |
| Concentration: | 0 μM, 25 μM, 50 μM, 75 μM, 100 μM |
| Incubation Time: | 48 hours |
| Result: | Exerted antiproliferative effects. |
体内研究(In Vivo)
Butylhydroxyanisole (200 mg/kg!i.g.!daily!for three days) induces distinct expression patterns of Nrf2 and detoxification enzymes in the liver and small intestine of C57BL/6 mice[3].
MCE has not independently confirmed the accuracy of these methods.They are for reference only.
| Animal Model: | Five-week-old C57BL/6 mice (WT and Nrf2-/-)[3] |
| Dosage: | 200 mg/kg |
| Administration: | Oral gavage, daily, for three days |
| Result: | Increased Nqo1 staining in hepatocytes, predominately in the pericentral region. |
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date | Phase |
|---|---|---|---|---|
| NCT05094817 | Women´s College Hospital|University Health Network, Toronto | February 11, 2022 | ||
| NCT03826784 | Carmell Therapeutics Corporation | November 2019 | Phase 3 | |
| NCT04056429 | Carmell Therapeutics Corporation | July 2020 | Phase 2 |
分子量：180.24
性状：Solid
Formula：C22H32O4
CAS 号：25013-16-5
中文名称：丁基羟基茴香醚；叔丁基对羟基茴香醚；丁基大茴醚
运输条件：Room temperature in continental US!may vary elsewhere.
储存方式
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
溶解性数据
DMSO : ≥ 100 mg/mL(554.82 mM)
H2O : 1 mg/mL(5.55 mM;ultrasonic and warming and heat to 60°C)
*"≥" means soluble, but saturation unknown.
| 浓度溶剂体积质量 | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 5.5482 mL | 27.7408 mL | 55.4816 mL |
| 5 mM | 1.1096 mL | 5.5482 mL | 11.0963 mL |
| 10 mM | 0.5548 mL | 2.7741 mL | 5.5482 mL |
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months!-20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂：
——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存；体内实验的工作液,建议您现用现配,当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶
-
1.
请依序添加每种溶剂： PBS
Solubility: 100 mg/mL (554.82 mM)!Clear solution!Need ultrasonic
参考文献
[1]. Sunwoo Park, et al.Butylated Hydroxyanisole Exerts Neurotoxic Effects by Promoting Cytosolic Calcium Accumulation and Endoplasmic Reticulum Stress in Astrocytes.J Agric Food Chem.2019 Aug 28;67(34):9618-9629.[Content Brief]
[2]. Jiyeon Ham, et al.Butylated Hydroxyanisole Exerts Neurotoxic Effects by Promoting Cytosolic Calcium Accumulation and Endoplasmic Reticulum Stress in Astrocytes.Sci Total Environ.2020 Feb 1;702:134775.[Content Brief]
[3]. Lin Luo, et al.Butylated hydroxyanisole induces distinct expression patterns of Nrf2 and detoxification enzymes in the liver and small intestine of C57BL/6 mice.Toxicol Appl Pharmacol.2015 Nov 1;288(3):339-48.[Content Brief]
[4]. Jennifer Yinuo Cao, et al.Mechanisms of ferroptosis.Cell Mol Life Sci.2016 Jun;73(11-12):2195-209.[Content Brief]
注：产品仅用于科研
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